POS-453 TO INVESTIGATE THE EFFECT OF HIRUDIN ON RENAL INTERSTITIAL INJURY AND CORE FUCOSYLATION INDUCED BY PROTEINURIA BASED ON MICROFLUIDICCHIPS

To investigate the protective effect of irudin on renal interstitial injury caused by proteinuria regulating core fucosylation modification using model microfluidic chip. Application tissue engineering soft lithography technology preparation with cavity proximal small pipe, tube Zhou Wei vascular lumen and area Polydimethylsiloxane chip material, in tubes each Human tubular epithelial cells umbilical vein endothelial cells,The vitro microenvironment "proximal tubules peritubular microvessels" was simulated. Then two kinds were inoculated model, experimental observation carried out after adhered to wall.The trained physical building used study cause damage development process tubule interaction mechanism blood vessels, experiment divided into three groups, normal control group, treatment group.The expression cell markers, fibrosis markers fucose transferase culture system under different conditions observed.Theexpressions FUT8, chain LCA, apoptosis Bax, Bcl2 Caspase3 detected immunofluorescence. (1) The collateral disease established technology, including microvessels composed HUVEC cells, HK-2 extracellular matrix between them. (2) After stimulating 5% serum, (3) hirudin could protect alleviate serum stimulation constructed technology. simulate proteinuria, reappear pathophysiological changes microvascular vitro, interstitium affecting modification.